Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
Experimental study of adjuvant effect of recombinant mycobacterial heat-shock protein rHSP70 on immune response to antigens of opportunistic microorganisms was performed. Therapeutic polycomponent vaccine Immunovac-VP-4, containing ligands for binding with Toll-like receptors (TLRs) 2, 4 and 9, was used as a source of bacterial antigens. Obtained data showed that administration of rHSP70 mixed with bacterial antigens of opportunistic microorganisms leads to maturation of mice dendritic cells obtained from bone marrow precursors, increased expression of TLRs 2, 4, 9 on their surface and production of cytokines IL-1β, IL-6, IL-10, TNFα, as well as to activation of innate immunity in experiments in vivo resulting to increased resistance of mice to experimental Salmonella infection. Simultaneous administration of rHSP70 with bacterial antigens increased titers of antibodies to vaccine’s antigenic components in direct hemagglutination reaction. Thus, adjuvant effect of rHSP70 was confirmed on the used model as well as increase of stimulation of innate and adaptive immunity was shown.
Zh. Mikrobiol. (Moscow), 2009, No. 1, P. 42—46